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Ánalysis of numeric abnormalities in chromosomes
1 and 7 and DNA ploidy in breast cancer
Kapranos N., Kouneli S., Karantasi E., Arvaniti H.,
Sava S., Patra F., Sfikas C., Kokka E., Bairaktari E.
Pathology Departments "Amalia Fleming" Gen. Hosp. Athens,
Maternity Hospital "H. Venizelou" Athens
AIM: To investigate numeric alterations of chromosomes
1 and 7 as well as DNA ploidy status in breast cancer and to correlate
the findings with parameters of prognostic significance.
MATERIAL AND METHODS: Fluorescence in situ hybridization
(FISH) with pericentromeric probes for chromosomes 1 and 7 and
DNA content measurement by image analysis based cytophotometry
were applied in interphase nuclei from fresh tissue imprints of
29 breast carcinomas.
RESULTS: The correlation between DNA ploidy and chromosomal
aberrations revealed significant association between aneuploidy
and simultaneous aneusomy of chromosomes 1 and 7 (P=0.0007). A
similar association was found for chromosome 1 alone (P=0.001).
A significant correlation was also demonstrated between regional
lymph node metastasis and aneusomy of both chromosomes as well
as chromosome 1 alone (P=0.02 and 0.05 respectively). Polysomy
of chromosomes 1 and 7 was more frequent in poorly differentiated
(grade III) tumors. No association between chromosome alterations
and tumor size, hormone receptor status or p53 protein expression
was detected.
CONCLUSION: Our findings indicate that the detection of
numerical aberrations of chromosomes 1 and 7 with the FISH technique
seems to be more sensitive for detection of abnormal DNA and their
presence might be a useful predictor of aggressive biological
behavior.
Key words: Breast carcinoma, Fluorescent in situ hybridization,
chromosomes 1 and 7, DNA ploidy.
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