Analysis of numeric abnormalities in chromosomes
1 and 7 and DNA ploidy in breast cancer
Kapranos N, Kouneli S, Karadasi E, Arvaniti E, Savva S, Patra
F, Sfikas K, Kokka E, Kouri-Bairaktari E.
Aim: To investigate numeric alterations of chromosomes
1 and 7 as well as DNA ploidy status in breast cancer and to correlate
the findings with parameters of prognostic significance.
Material and Methods: Fluorescence in situ hybridization
(FISH) with pericentromeric probes for chromosomes 1 and 7 and
DNA content measurement by image analysis based cytophotometry
were applied in interphase nuclei from fresh tissue imprints of
29 breast carcinomas.
Results: The correlation between DNA ploidy and chromosomal
aberrations revealed signifcant association between aneuploidy
and simultaneous aneusomy of chromosomes 1 and 7 (P=0.0007). A
similar association was found for chromosome 1 alone (P=0.001).
A significant correlation was also demonstrated between regional
lymph node metastasis and aneusomy of both chromosomes as well
as chromosome 1 alone (P=0.02 and 0.05 respectively). Polysomy
of chromosomes 1 and 7 was more frequent in poorly differentiated
(grade III) tumors. No association between chromosome alterations
and tumor size, hormone receptor status or p53 protein expression
was detected.
Conclusion: Our findings indicate that the detection of
numerical aberrations of chromosomes 1 and 7 with the FISH technique
seems to be more sensitive for detection of abnormal DNA and their
presence might be a useful predictor of aggressive biological
behavior.
Key words: FISH, DNA Ploidy, Breast cancer, chromosomes
1,7.
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