Ovarian sex cord-stromal tumors:
A fluoresence in situ hybridization study
of chromosomes 12 and 7
Kostopoulou E., Agelidou S., Leontsini M.
Hippokrateion Hospital of Thessaloniki, Pathology Department
Aim: The aim of the present study was: a) to
examine the presence of trisomy 12 in ovarian sex cord-stromal
tumors by fluoresence in situ hybridization (F.I.S.H.), b) to
examine the presence of correlations between trisomy 12 in fibrothecomas
and patient age, tumor size, or Ki67 labelling index, and c) to
examine granulosa and Sertoli-Leydig cell tumors for the presence
of trisomy 7.
Material and methods: 26 tumors from our files
have been studied by F.I.S.H. (6 fibromas, 9 thecomas, 5 granulosa
cell tumors, 2 sclerosing stromal tumors, 4 Sertoli-Leydig cell
tumors). a-satellite probes for chromosomes 12 and 7 have been
used. The fibromas and thecomas were stained immunohistochemically
for Ki67.
Results: Trisomy 12 was observed in 10/15 fibromas-thecomas
(9-40% of the nuclei), 2/5 granulosa cell tumors (10-35% of the
nuclei) and 2 sclerosing stromal tumors (20-30% of the nuclei),
while it was not observed in Sertoli-Leydig cell tumors. High
percentage of trisomic nuclei (>15%) was found in fibromas and
thecomas of larger size (p<0.05). Ki67 labelling index (LI) of
fibromas and thecomas was low, ranging from 0.5 to 3%. There was
no correlation between trisomy 12 and Ki67-LI or patient age.
Trisomy 7 was observed only in one granulosa cell tumor.
Conclusions: Trisomy 12 is a common finding
in granulosa - stromal cell tumors, although the percentage varies
in different studies. Its common presence may suggest a degree
of similarity in the evolution of histologically different neoplasms
of the above group.
Key words: Īvarian tumors, fibrothecoma, granulosa cell tumor, Sertoli-Leydig
cell tumor, sclerosing stromal tumor, trisomy 12, fluoresence
in situ hybridization.
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