|
Hellenic Archives of Pathology, Volume 20, Issues 1-3, 2006
An easy and reliable method
for the preparation of semi-thin tissue sections
in resin and the subsequent histochemical
and immunohistochemical staining
Alexiou Ì., Patra É., Batistatou Á.
Department of Pathology, University Hospital of Ioannina, Greece
The preparation of semi-thin tissue sections from resin-embedded material is a particularly useful technique in Pathology laboratories for diagnostic purposes and research protocols. In our laboratory we have utilized this method in order to study the regeneration of the peroneal nerve after transection and suture. In addition, we have strengthened our results with the application of immunohistochemical technique after removal of the resin. In this study, we present the modifications we have applied in the classical method of preparation of semi-thin tissue sections in resin, as well as a simple histochemical stain and an immunohistochemical technique. The important-innovative steps are the removal of resin with a saturated buffer of NaOH in ethanol, the staining of the tissue sections with a very diluted Toluidine Blue stain (0.3%) and the subsequent immunostaining of section with an anti-S100 antibody.
Key words: Resin, semi-thin sections, histochemistry, immunohistochemistry.
|
